FEBS letters · 2024
The splicing factor hnRNPL demonstrates conserved myocardial regulation across species and is altered in heart failure.
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Draper Isabelle, Huang Wanting, Pande Suchita, Zou Aaron, Calamaras Timothy D, Choe Richard H, Correia-Branco Ana, Mei Ariel L, Chen Howard H, Littel Hannah R, Gunasekaran Mekala, Wells Natalya M, Bruels Christine C, Daugherty Audrey L, Wolf Matthew J, Kang Peter B, Yang Vicky K, Slonim Donna K, Wallingford Mary C, Blanton Robert M
Аннотация
Heart failure (HF) is highly prevalent. Mechanisms underlying HF remain incompletely understood. Splicing factors (SF), which control pre-mRNA alternative splicing, regulate cardiac structure and function. This study investigated regulation of the splicing factor heterogeneous nuclear ribonucleoprotein-L (hnRNPL) in the failing heart. hnRNPL protein increased in left ventricular tissue from mice with transaortic constriction-induced HF and from HF patients. In left ventricular tissue, hnRNPL was detected predominantly in nuclei. Knockdown of the hnRNPL homolog Smooth in Drosophila induced cardiomyopathy. Computational analysis of predicted mouse and human hnRNPL binding sites suggested hnRNPL-mediated alternative splicing of tropomyosin, which was confirmed in C2C12 myoblasts. These findings identify hnRNPL as a sensor of cardiac dysfunction and suggest that disturbances of hnRNPL affect alternative splicing in HF.