[Application of the periodic action method to studying the kinetics of carbon monoxide binding by myoglobin].

To investigate the enzymatic and chemical reactions in a regime of forced oscillations an apparatus has been constructed which permits to observe amplitude-frequency and phase-frequency characteristics of objects in the range of frequencies 10-2-10-6 c/s. This apparatus offers a means of recording stationary and transition kinetics of reactions after the jump-perturbation. Reaction kinetics of CO-binding by whale myoglobin has been studied in the regime of forced oscillations. A phase-frequency characteristics of the system and frequency dependence of averaged (for many periods) myoglobin concentration is measured. Each of the two relationships permits to measure the relaxation times. This possibility has been theoretically predicted earlier by the author.