Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi · 2009
[Study on the feasibility of a cell-culture inoculation test in the detection and isolation of street rabies virus].
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Wang Xia, Xu Ge-Lin, Wu Jie, Luo Jing, Zhu Jia-Hong, Zhou Jian-Jun, Shi Nan-Feng, Tang Qing, Zhang Yong-Zhen, Tang Jian-Rong, Wu Tai-Cai, Ming He-Tian, Shen Rui-Hua, Chen Huan-Chun
Аннотация
OBJECTIVE: Feasibility of using MNA cell-culture inoculation test to detect and isolate the street rabies virus.
METHODS: Using MNA cell-culture inoculation test, fluorescent antibody test (FAT) and sandwich ELISA with double-antibodies to detect 33 specimens of street rabies virus, 20 specimens of negative canine brains and 4 specimens of healthy mice brains.
RESULTS: 33 specimens of street rabies virus were positive to the cell-culture inoculation test but the others were negative. The concordances of MNA cell-cultured inoculation test with FAT and sandwich ELISA with double-antibodies were both 100%.
CONCLUSION: MNA cell-culture inoculation test appeared to be both highly sensitive and specific in detecting the street rabies virus, and could be used in detection and isolation of the virus.
METHODS: Using MNA cell-culture inoculation test, fluorescent antibody test (FAT) and sandwich ELISA with double-antibodies to detect 33 specimens of street rabies virus, 20 specimens of negative canine brains and 4 specimens of healthy mice brains.
RESULTS: 33 specimens of street rabies virus were positive to the cell-culture inoculation test but the others were negative. The concordances of MNA cell-cultured inoculation test with FAT and sandwich ELISA with double-antibodies were both 100%.
CONCLUSION: MNA cell-culture inoculation test appeared to be both highly sensitive and specific in detecting the street rabies virus, and could be used in detection and isolation of the virus.