Wei sheng wu xue bao = Acta microbiologica Sinica · 2009
[Reverse genetic system for rabies virus vaccine Evelyn-Rokitnicki-Abelseth strain].
Оценочное исследованиеСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Guo Li, Feng Na, Yang Songtao, Wang Xijun, Ge Jinying, Xia Xianzhu, Bu Zhigao
Аннотация
OBJECTIVE: To establish a reverse genetic system of rabies virus for producing safe and efficient rabies vaccine.
METHOD: By reverse genetic and molecular cloning technique, we established two rabies virus rescue systems including (1) help plasmids expressing N, P and L protein, and (2) CMV/T7 and T7 promoter.
RESULTS: Wild-type rERA-VC was rescued by both systems, and had the same growth kinetics as parental virus. The third generation virus could grow to high titer.
CONCLUSION: The established reverse genetic system for rescuing wild-type rERA-VC provides the possibility of producing safe and efficient rabies virus vaccines.
METHOD: By reverse genetic and molecular cloning technique, we established two rabies virus rescue systems including (1) help plasmids expressing N, P and L protein, and (2) CMV/T7 and T7 promoter.
RESULTS: Wild-type rERA-VC was rescued by both systems, and had the same growth kinetics as parental virus. The third generation virus could grow to high titer.
CONCLUSION: The established reverse genetic system for rescuing wild-type rERA-VC provides the possibility of producing safe and efficient rabies virus vaccines.