Proceedings of the National Academy of Sciences of the United States of America · 2023
In situ architecture and membrane fusion of SARS-CoV-2 Delta variant.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Song Yutong, Yao Hangping, Wu Nanping, Xu Jialu, Zhang Zheyuan, Peng Cheng, Li Shibo, Kong Weizheng, Chen Yong, Zhu Miaojin, Wang Jiaqi, Shi Danrong, Zhao Chongchong, Lu Xiangyun, Echavarría Galindo Martín, Li Sai
Аннотация
Among the current five Variants of Concern, infections caused by SARS-CoV-2 B.1.617.2 (Delta) variant are often associated with the greatest severity. Despite recent advances on the molecular basis of elevated pathogenicity using recombinant proteins, the architecture of intact Delta virions remains veiled. Moreover, pieces of molecular evidence for the detailed mechanism of S-mediated membrane fusion are missing. Here, we showed the pleomorphic nature of Delta virions from electron beam inactivated samples and reported the in situ structure and distribution of S on the authentic Delta variant. We also captured the virus-virus fusion events, which provided pieces of structural evidence for Delta's attenuated dependency on cellular factors for fusion activation, and proposed a model of S-mediated membrane fusion. Besides, site-specific glycan analysis revealed increased oligomannose-type glycosylation of native Delta S than that of the WT S. Together, these results disclose distinctive factors of Delta being the most virulent SARS-CoV-2 variant.