Integrating scanned literature and online sources to build a data text collection for plant biodiversity.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
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Loberg Matthew A, Xu George J, Chen Sheau-Chiann, Chen Hua-Chang, Wahoski Claudia C, Caroland Kailey P, Tigue Megan L, Hartmann Heather A, Gallant Jean-Nicolas, Phifer Courtney J, Ocampo Andres A, Wang Dayle K, Fankhauser Reilly G, Karunakaran Kirti A, Wu Chia-Chin, Tarabichi Maxime, Shaddy Sophia M, Netterville James L, Rohde Sarah L, Solórzano Carmen C, Bischoff Lindsay A, Baregamian Naira, Murphy Barbara A, Choe Jennifer H, Wang Jennifer R, Huang Eric C, Sheng Quanhu, Kagohara Luciane T, Jaffee Elizabeth M, Belcher Ryan H, Lau Ken S, Ye Fei, Lee Ethan, Weiss Vivian L
Аннотация
Although well-differentiated thyroid carcinoma (WDTC) is characterized by a robust treatment response, aggressive subtypes, such as anaplastic thyroid carcinoma (ATC), remain highly lethal. To understand thyroid cancer evolution in both children and adults, we analyzed single-cell transcriptomes of 423,733 cells from 81 samples and spatially resolved key tumor and microenvironment populations across 28 tumors with spatial transcriptomics, including rare and unique composite WDTC/ATC tumors and pediatric diffuse sclerosing thyroid carcinomas. Additionally, we identified gene signatures of stromal cell populations in 5 large thyroid cancer bulk RNA-sequencing cohorts. Through this multi-institutional effort, we defined a population of POSTN+ myofibroblast cancer-associated fibroblasts (myCAFs) that are intimately associated with invasive tumor cells and correlate with poor prognosis, lymph node metastasis, and disease progression in thyroid carcinoma. We also revealed a population of inflammatory CAFs that are distant to tumor cells and are found in the inflammatory stromal microenvironment of autoimmune thyroiditis. Together, our study provides spatial profiling of thyroid cancer evolution in samples with mixed WDTC/ATC histopathology and identifies a prognostic myCAF subtype with potential clinical utility in predicting aggressive disease in both children and adults.