Journal of biomedical optics · 2025
Activity of antimicrobial photodynamic therapy against a cariogenic biofilm composed of a microbial consortium.
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Thurler Bruna Alves, Santos Thamyris Py Domingos Faial, Motta Paula Carvalho, Pereira Gabriella Lorena Dias, Chianca Gabriela Ceccon, Povoa Helvecio Cardoso Correa, Fontes Karla Bianca Fernandes da Costa, Iorio Natalia Lopes Pontes Povoa
Аннотация
SIGNIFICANCE: Dental caries is a polymicrobial condition derived from microbial biofilm. There is a lack of studies addressing antimicrobial photodynamic therapy (aPDT) activity against a cariogenic multispecies biofilm.
AIM: We aim to evaluate the activity of aPDT against a cariogenic biofilm composed of a microbial consortium.
APPROACH: Equal parts of Streptococcus mutans, Lactobacillus rhamnosus, and Candida albicans were used to form a microbial inoculum containing ∼ 10 7 colony-forming units/mL, which was placed on cellulose acetate membranes to form biofilm. After biofilm formation, the seven groups, each containing four membranes, were treated as follows: laser 1 J (G1); laser 4 J (G2); photosensitizer methylene blue (G3); photosensitizer + laser 1 J (G4); photosensitizer + laser 4 J (G5); chlorhexidine as positive control (G6); and distilled water (G7).
RESULTS: The number of viable microbial cells per biofilm varied between 1.40 × 10 8 (G5) and 7.28 × 10 8 (G1), whereas the negative control group (G7) reached 1.38 × 10 9 . Compared with G7, all groups presented a reduction, with the percentage varying from 47.05% (G1) to 89.85% (G5). However, G5 (photosensitizer + laser 4 J) was the only group to present a statistical reduction ( p < 0.05 ).
CONCLUSION: aPDT represents an important antibiofilm adjunct therapy, resulting in a significant reduction in microbial cells within a cariogenic biofilm model.
AIM: We aim to evaluate the activity of aPDT against a cariogenic biofilm composed of a microbial consortium.
APPROACH: Equal parts of Streptococcus mutans, Lactobacillus rhamnosus, and Candida albicans were used to form a microbial inoculum containing ∼ 10 7 colony-forming units/mL, which was placed on cellulose acetate membranes to form biofilm. After biofilm formation, the seven groups, each containing four membranes, were treated as follows: laser 1 J (G1); laser 4 J (G2); photosensitizer methylene blue (G3); photosensitizer + laser 1 J (G4); photosensitizer + laser 4 J (G5); chlorhexidine as positive control (G6); and distilled water (G7).
RESULTS: The number of viable microbial cells per biofilm varied between 1.40 × 10 8 (G5) and 7.28 × 10 8 (G1), whereas the negative control group (G7) reached 1.38 × 10 9 . Compared with G7, all groups presented a reduction, with the percentage varying from 47.05% (G1) to 89.85% (G5). However, G5 (photosensitizer + laser 4 J) was the only group to present a statistical reduction ( p < 0.05 ).
CONCLUSION: aPDT represents an important antibiofilm adjunct therapy, resulting in a significant reduction in microbial cells within a cariogenic biofilm model.