European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2024
Development and evaluation of a loop-mediated isothermal amplification (LAMP) method for Candida glabrata detection.
СтатьяОценочное исследованиеНазвание и аннотация: Перевод пока не подготовлен
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Yahaya H, Cheah Y K, Chee H Y, Than L T L
Аннотация
PURPOSE: Loop-mediated isothermal amplification (LAMP) is a simple and rapid nucleic acid method for DNA amplification at a constant temperature. The "gold standard" culture method for yeast detection, has low sensitivity with severe consequences, increasing morbidity and mortality rates. Here, we report the development of a LAMP method for the specific detection of C. glabrata.
METHODOLOGY: The specific LAMP primers for C. glabrata detection were designed and evaluated.
RESULTS: The LAMP assay accurately detected C. glabrata with no cross-reactivity with other Candida species.
CONCLUSION: The developed molecular method would be a promising tool in the management of invasive candidiasis.
METHODOLOGY: The specific LAMP primers for C. glabrata detection were designed and evaluated.
RESULTS: The LAMP assay accurately detected C. glabrata with no cross-reactivity with other Candida species.
CONCLUSION: The developed molecular method would be a promising tool in the management of invasive candidiasis.