[Study on effect of glycidyl methacrylate on cell cycle and apoptosis in human bronchial epithelial cells].

OBJECTIVE

To investigate the effect of glycidyl methacrylate (GMA) on cell cycle and apoptosis in human bronchial epithelial cells.

METHODS

Flow cytometry was applied to determine cell cycle and apoptosis of 16HBE cells treated at the doses of 1-16 microg/ml GMA for different time. Mitotic index (MI) of cell was also analysed.

RESULTS

When 16HBE cells were treated by GMA for 72hr, cells in G0/G1 phase decreased, but cells in S and G2/M phase increased. The increases of apoptosis rates and the decreases of mitotic index could also be observed. As treatment went on, all groups cells were stopped at G0/G1 phase, but at the dose of 16 microg/ml the cell increases of S and G2/M phase were observed in comparison with the controls. After cells were treated for 3 times, the decreases apoptosis rates and the increases of mitotic index of cell treated by high dosage were observed.

CONCLUSION

The 16HBE cells treated by GMA could result in cell cycle transition from G0/G1 phase to S and G2/M phase. It was suggested that the cell proliferation was stimulated. Uncontrolled cellular proliferation may be responsible for malignant transformation induced by GMA.