European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2025
Isolation and characterization of a lytic phage and its efficacy against multi-drug resistant strains of pseudomonas aeruginosa.
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Sarmast Mohammad, Peeri-Dogaheh Hadi, Karami Chiman, Khademi Farzad
Аннотация
BACKGROUND: Phages are potent alternatives to antibiotics due to their lytic activity and specificity. We isolated and characterized a specific bacteriophage effective against resistant strains of P. aeruginosa and their biofilms.
METHODS: Samples were collected from hospital sewage. The morphological and life-cycle characteristics, pH and thermal stability, and host range of the isolated phage were assessed. Additionally, the sensitivity of 42 P. aeruginosa strains and their biofilms was evaluated.
RESULTS: Morphological analysis showed that vB-pae-PA01 resembled Podophages. Sensitivity assays revealed that vB-pae-PA01 lysed 57% of the strains. vB-pae-PA01 exhibited strong thermal stability. However, highly acidic and basic conditions reduced its lytic activity. The one-step growth curve revealed a latent period of 10 min and a burst size of 105 PFU per cell.
CONCLUSION: We isolated the lytic bacteriophage vB-pae-PA01, demonstrating potent lytic activity against P. aeruginosa strains. These properties suggest that vB-pae-PA01 could be a potential candidate to use against antibiotic-resistant P. aeruginosa.
METHODS: Samples were collected from hospital sewage. The morphological and life-cycle characteristics, pH and thermal stability, and host range of the isolated phage were assessed. Additionally, the sensitivity of 42 P. aeruginosa strains and their biofilms was evaluated.
RESULTS: Morphological analysis showed that vB-pae-PA01 resembled Podophages. Sensitivity assays revealed that vB-pae-PA01 lysed 57% of the strains. vB-pae-PA01 exhibited strong thermal stability. However, highly acidic and basic conditions reduced its lytic activity. The one-step growth curve revealed a latent period of 10 min and a burst size of 105 PFU per cell.
CONCLUSION: We isolated the lytic bacteriophage vB-pae-PA01, demonstrating potent lytic activity against P. aeruginosa strains. These properties suggest that vB-pae-PA01 could be a potential candidate to use against antibiotic-resistant P. aeruginosa.