Traditional formulation-Vanari Gutika treatment improves the testosterone biosynthesis and sperm quality in adult male mice.

OBJECTIVES

Vanari Gutika (VG), an Indian traditional formulation containing Mucuna pruriens as a principal component, is traditionally used for the management of male reproductive disorders. However, there is a significant lack of evidence-based research validating its effects on male fertility and testosterone biosynthesis. This study aimed to investigate the impact of VG on testicular steroidogenesis, lipid peroxidation, and sperm quality in male mice.

METHODS

Adult male mice were orally administered VG at 75, 150, or 300 mg/kg body weight/day for 35 days. Testicular function was assessed through sperm morphology and viability, lipid peroxidation and expression of key steroidogenic and oxidative stress-related proteins.

RESULTS

VG at 150 and 300 mg/kg significantly increased testis weight and serum testosterone, with a concurrent reduction in estradiol. VG (150 mg/kg) improved normal sperm morphology and reduced abnormalities. Markers of oxidative stress status improved, evidenced by reduced LPO and increased Nrf-2 expression. VG treatment enhanced the expression of steroidogenic markers (SF-1, CYP11A1, 3β-HSD, StAR, and 17β-HSD) at higher doses.

CONCLUSIONS

Higher doses of VG (150 and 300 mg/kg BW) promote testicular androgenesis, reduces oxidative stress, and improves sperm quality. Consequently, our results provide robust and compelling evidence supporting its potential as a dietary supplement for boosting the testosterone level and sperm quality.