Molecular biology reports · 2025
JADE1 is not essential for spermatogenesis and male fertility in mice.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Zhu Yucong, Cao Huiwen, Yu Chao
Аннотация
BACKGROUND: Histone acetylation is essential for histone-to-protamine transition during spermiogenesis. JADE1 contributes to the assembly of the JADE1-HBO1 complex and facilitates HBO1-mediated histone acetylation. However, whether the JADE1-HBO1 complex regulates histone acetylation in spermiogenesis is unknown.
METHODS AND RESULTS: In this study, we generated a germ cell-specific Jade1 conditional knockout mouse (Jade1 cKO) and analysed the spermatogenesis and fertility. Despite Jade1 cKO males exhibiting reduced testes weight, histological analysis and nuclear surface spreading revealed that spermatogenesis in Jade1 cKO mice remained unaffected. Jade1 cKO males also showed normal count, morphology, and fertility of the spermatozoa. Furthermore, deletion of JADE1 did not affect the dynamics of H4K16ac and protein levels of core histones (H2A, H2B, H3, and H4) during spermiogenesis in mice.
CONCLUSIONS: Based on these findings, we infer that JADE1 is dispensable for the dynamics of H4K16ac or core histone degradation during spermiogenesis, and is not essential for male fertility. Our findings provide new insights for further investigation of the biological functions of JADE1 in the murine model.
METHODS AND RESULTS: In this study, we generated a germ cell-specific Jade1 conditional knockout mouse (Jade1 cKO) and analysed the spermatogenesis and fertility. Despite Jade1 cKO males exhibiting reduced testes weight, histological analysis and nuclear surface spreading revealed that spermatogenesis in Jade1 cKO mice remained unaffected. Jade1 cKO males also showed normal count, morphology, and fertility of the spermatozoa. Furthermore, deletion of JADE1 did not affect the dynamics of H4K16ac and protein levels of core histones (H2A, H2B, H3, and H4) during spermiogenesis in mice.
CONCLUSIONS: Based on these findings, we infer that JADE1 is dispensable for the dynamics of H4K16ac or core histone degradation during spermiogenesis, and is not essential for male fertility. Our findings provide new insights for further investigation of the biological functions of JADE1 in the murine model.