Current eye research · 2011
Long-term topical antiglaucoma medications cause enhanced Tenon's capsule fibroblast proliferation and abnormal TGF-β and MMP expressions: potential effects on glaucoma filtering surgery.
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Leng Fei, Liu Ping, Li Hulun, Zhang Jiaying
Аннотация
PURPOSE: To investigate the changes in Tenon's capsule fibroblast (HTF) proliferation and their expressions of transforming growth factor-β (TGF-β) and matrix metalloproteinases (MMPs) in patients with long-term use of topical antiglaucoma medications, and to evaluate the potential effect of these medications on the outcome of glaucoma filtering surgery for primary open-angle glaucoma (POAG).
METHODS: HTFs isolated from the conjunctiva of ten POAG patients and ten control patients with cataract or retinal detachment were tested for their in vitro proliferation capacity. RT-PCR (reverse-transcription polymerase chain reaction) was performed to detect the expressions of TGF-β and MMP mRNAs in the HTFs, and the expression of TGF-β2 protein was detected immunohistochemically. Enzyme-linked immunosorbent assay (ELISA) was used to detect the expression of TGF-β1 in the supernatant of the HTFs treated with the topical antiglaucoma agents.
RESULTS: The HTFs from the POAG patients showed significantly higher proliferative activity than those from the control patients (P = 0.0013). The HTFs in the POAG group showed significantly higher expressions of TGF-β2 and TGF-β3 mRNA and lower MMP-9 mRNA than the control HTFs. MMP-3 mRNA was not detected in the HTFs in POAG groups, but lowly expressed in the control HTFs. The HTFs in the POAG group had a significantly higher expression of TGF-β2 protein; TGF-β1 protein was not detected in the control HTFs, but was highly expressed in the POAG group. Treatment of the HTFs with latanoprost resulted in a 4-fold increase in TGF-β1 expression in both groups, which was comparable for the expression after the exposure.
CONCLUSIONS: Long-term use of some topical antiglaucoma medications might be associated with enhanced HTF proliferation and abnormal expressions of TGF-β and MMPs, which might have promoted scarring of the filtering blebs following glaucoma filtering surgery.
METHODS: HTFs isolated from the conjunctiva of ten POAG patients and ten control patients with cataract or retinal detachment were tested for their in vitro proliferation capacity. RT-PCR (reverse-transcription polymerase chain reaction) was performed to detect the expressions of TGF-β and MMP mRNAs in the HTFs, and the expression of TGF-β2 protein was detected immunohistochemically. Enzyme-linked immunosorbent assay (ELISA) was used to detect the expression of TGF-β1 in the supernatant of the HTFs treated with the topical antiglaucoma agents.
RESULTS: The HTFs from the POAG patients showed significantly higher proliferative activity than those from the control patients (P = 0.0013). The HTFs in the POAG group showed significantly higher expressions of TGF-β2 and TGF-β3 mRNA and lower MMP-9 mRNA than the control HTFs. MMP-3 mRNA was not detected in the HTFs in POAG groups, but lowly expressed in the control HTFs. The HTFs in the POAG group had a significantly higher expression of TGF-β2 protein; TGF-β1 protein was not detected in the control HTFs, but was highly expressed in the POAG group. Treatment of the HTFs with latanoprost resulted in a 4-fold increase in TGF-β1 expression in both groups, which was comparable for the expression after the exposure.
CONCLUSIONS: Long-term use of some topical antiglaucoma medications might be associated with enhanced HTF proliferation and abnormal expressions of TGF-β and MMPs, which might have promoted scarring of the filtering blebs following glaucoma filtering surgery.
Предметные рубрики MeSH
Administration, TopicalAntihypertensive AgentsCell ProliferationCells, CulturedConjunctivaEnzyme-Linked Immunosorbent AssayFemaleFibroblastsFiltering SurgeryFollow-Up StudiesGlaucoma, Open-AngleHumansImmunoenzyme TechniquesMaleMatrix MetalloproteinasesMiddle AgedRNA, MessengerReverse Transcriptase Polymerase Chain ReactionTenon CapsuleTransforming Growth Factor beta