Academic radiology · 2025
Quantitation of Acute Extremity Compartment Syndrome in Rabbits Using Shear-wave Elastography to Assess Viscoelasticity.
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Ren Xiaobo, Zhang Yiqing, Zhai Peng, Sun Lei, Li Zhenzhou, Chen Xin, Lu Minhua
Аннотация
RATIONALE AND OBJECTIVES: Acute extremity compartment syndrome (AECS) lacks reliable noninvasive diagnostics. This study aimed to validate shear-wave elastography-derived viscoelastic parameters for assessing muscle pathology progress in a rabbit AECS model.
METHODS: Forty-eight New Zealand rabbits were randomized into eight groups (control, 4, 6, 8, 12, 24, 48, and 72 h post-procedure; n = 6). AECS was induced via ultrasound-guided femoral artery hypoperfusion (75% flow reduction) and calf cuff compression (200 mmHg, 2 h). Shear-wave speed (SWS) and viscosity (η) of the triceps surae muscle were measured using a programmable ultrasound, with intra-compartmental pressure (ICP) monitored concurrently. Histopathological grading (HGS: 0-9 scale) and ultrastructural staging (USS: 0-4 scale) were performed on hematoxylin and eosin (H&E) or transmission electron microscope (TEM) sections. Apoptosis (TUNEL apoptotic index) and systemic inflammation (plasma TNF-α) were quantified.
RESULTS: Histology showed inflammatory cell infiltration, interstitial edema, necrosis, and microenvironmental degeneration in model limbs. HGS strongly correlated with SWS (r = 0.693) and η (r = 0.710, both p < 0.001). η was strongly associated with TNF-α (r = 0.702) and necrosis (r = 0.722), while SWS weakly correlated with inflammation (r = 0.345, p = 0.029). Receiver operating characteristic (ROC) analysis revealed η outperformed SWS in lesions detection (AUC: 0.894 [95% CI: 0.832-0.955] vs. 0.829 [0.749-0.908], p > 0.05). Combined SWS-η metrics improved diagnostic accuracy (AUC = 0.905 [0.845-0.965], p = 0.007 vs. SWS alone).
CONCLUSION: SWS and η effectively quantify necrosis and inflammation in AECS progression and offer potential for noninvasive, continuous monitoring.
METHODS: Forty-eight New Zealand rabbits were randomized into eight groups (control, 4, 6, 8, 12, 24, 48, and 72 h post-procedure; n = 6). AECS was induced via ultrasound-guided femoral artery hypoperfusion (75% flow reduction) and calf cuff compression (200 mmHg, 2 h). Shear-wave speed (SWS) and viscosity (η) of the triceps surae muscle were measured using a programmable ultrasound, with intra-compartmental pressure (ICP) monitored concurrently. Histopathological grading (HGS: 0-9 scale) and ultrastructural staging (USS: 0-4 scale) were performed on hematoxylin and eosin (H&E) or transmission electron microscope (TEM) sections. Apoptosis (TUNEL apoptotic index) and systemic inflammation (plasma TNF-α) were quantified.
RESULTS: Histology showed inflammatory cell infiltration, interstitial edema, necrosis, and microenvironmental degeneration in model limbs. HGS strongly correlated with SWS (r = 0.693) and η (r = 0.710, both p < 0.001). η was strongly associated with TNF-α (r = 0.702) and necrosis (r = 0.722), while SWS weakly correlated with inflammation (r = 0.345, p = 0.029). Receiver operating characteristic (ROC) analysis revealed η outperformed SWS in lesions detection (AUC: 0.894 [95% CI: 0.832-0.955] vs. 0.829 [0.749-0.908], p > 0.05). Combined SWS-η metrics improved diagnostic accuracy (AUC = 0.905 [0.845-0.965], p = 0.007 vs. SWS alone).
CONCLUSION: SWS and η effectively quantify necrosis and inflammation in AECS progression and offer potential for noninvasive, continuous monitoring.