Future microbiology · 2019
Modulatory effects of verapamil in rifampicin activity against Mycobacterium tuberculosis.
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Caleffi-Ferracioli Katiany R, Cardoso Rosilene F, de Souza João Vp, Murase Letícia S, Canezin Pedro H, Scodro Regiane Bl, Ld Siqueira Vera, Pavan Fernando R
Аннотация
AIM: To evaluate modulatory effect of verapamil (VP) in rifampicin (RIF) activity and its effect in efflux pumps (EPs) transcript levels in Mycobacterium tuberculosis.
MATERIALS & METHODS: RIF and VP minimal inhibitory concentration, combinatory effect and detection of mutations were determined in 16 isolates. EPs transcript levels were determined in four isolates by real-time PCR after exposure to drugs.
RESULTS: VP showed good combinatory effect among RIF-resistant isolates. This effect was also observed in the relative transcript levels of EPs, mainly after 72 h of exposure, depending on the EP gene, genotype and the resistance profile of the isolate.
CONCLUSION: Additional regulatory mechanisms in the EP activities, as well as, interactions with other drug-specific resistance mechanisms need further investigation in M. tuberculosis.
MATERIALS & METHODS: RIF and VP minimal inhibitory concentration, combinatory effect and detection of mutations were determined in 16 isolates. EPs transcript levels were determined in four isolates by real-time PCR after exposure to drugs.
RESULTS: VP showed good combinatory effect among RIF-resistant isolates. This effect was also observed in the relative transcript levels of EPs, mainly after 72 h of exposure, depending on the EP gene, genotype and the resistance profile of the isolate.
CONCLUSION: Additional regulatory mechanisms in the EP activities, as well as, interactions with other drug-specific resistance mechanisms need further investigation in M. tuberculosis.
Предметные рубрики MeSH
Antitubercular AgentsBacterial ProteinsBrazilCatalaseDNA-Directed RNA PolymerasesDrug Resistance, Multiple, BacterialDrug SynergismDrug Therapy, CombinationGene Expression Regulation, BacterialGenotypeHumansMembrane Transport ProteinsMicrobial Sensitivity TestsMutationMycobacterium tuberculosisOxidoreductasesRifampinTime FactorsTuberculosis, Multidrug-ResistantVerapamil