Revista da Sociedade Brasileira de Medicina Tropical · 2018
PCR sensitivity of peripheral blood of dogs co-infected with Leishmania spp. and Ehrlichia spp. in endemic area of Brazil.
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Silveira Ana Paula Stefanello da, Vieira Victor Bruno Duarte, Batalini Leticia Surian, Carmo Silvia Barbosa do, Friozi Elisabete, Arruda Eduardo José de, Lima Junior Manoel Sebastião da Costa, Neitzke-Abreu Herintha Coeto
Аннотация
INTRODUCTION: Peripheral blood of 400 dogs infected with Leishmania and Ehrlichia were analyzed using polymerase chain reaction (PCR), and clinical signs were characterized.
METHODS: PCR and parasitological tests were conducted.
RESULTS: PCR was positive for Leishmania in 84.75%, and parasitological tests showed that 63.25% and 31.75% were positive for Leishmania and Ehrlichia, respectively. All animals showed more than three clinical signs. PCR results were negative for Leishmania in 15.25% of the samples.
CONCLUSIONS: Conventional PCR of peripheral blood can be used for diagnosing canine visceral leishmaniasis in combination with other techniques, especially in uncertain cases that need species identification.
METHODS: PCR and parasitological tests were conducted.
RESULTS: PCR was positive for Leishmania in 84.75%, and parasitological tests showed that 63.25% and 31.75% were positive for Leishmania and Ehrlichia, respectively. All animals showed more than three clinical signs. PCR results were negative for Leishmania in 15.25% of the samples.
CONCLUSIONS: Conventional PCR of peripheral blood can be used for diagnosing canine visceral leishmaniasis in combination with other techniques, especially in uncertain cases that need species identification.