Chinese medical journal · 2003
Local tissue hypoxia and formation of nasal polyps.
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Jiang Shu, Dong Zhen, Zhu Dongdong, Yang Zhanquan
Аннотация
OBJECTIVE: To explore the response of nasal mucosa epithelial cells to hypoxia in terms of formation of nasal polyps (NP).
METHODS: Epithelial cells of NP and inferior turbinate (IT) were cultured serum-free under normal oxygen and hypoxic circumstances with stimulation of IL-1 beta and TNF alpha. The vascular endothelial growth factor (VEGF) mRNA and VEGF protein levels of the cultured cells were detected using in situ hybridization and ELISA, respectively.
RESULTS: The expression of VEGF mRNA was significantly higher in epithelial cells of NP than in IT exposed to pro-inflammatory cytokines or hypoxia (P < 0.01). VEGF levels were higher in NP epithelial cells than those of IT (P < 0.01) under hypoxia.
CONCLUSION: VEGF-induced by hypoxia is very important for the early stages of forming polyps.
METHODS: Epithelial cells of NP and inferior turbinate (IT) were cultured serum-free under normal oxygen and hypoxic circumstances with stimulation of IL-1 beta and TNF alpha. The vascular endothelial growth factor (VEGF) mRNA and VEGF protein levels of the cultured cells were detected using in situ hybridization and ELISA, respectively.
RESULTS: The expression of VEGF mRNA was significantly higher in epithelial cells of NP than in IT exposed to pro-inflammatory cytokines or hypoxia (P < 0.01). VEGF levels were higher in NP epithelial cells than those of IT (P < 0.01) under hypoxia.
CONCLUSION: VEGF-induced by hypoxia is very important for the early stages of forming polyps.
Предметные рубрики MeSH
Cell HypoxiaCells, CulturedEndothelial Growth FactorsEnzyme-Linked Immunosorbent AssayErythropoietinHumansIntercellular Signaling Peptides and ProteinsInterleukin-1LymphokinesNasal MucosaNasal PolypsRNA, MessengerTumor Necrosis Factor-alphaVascular Endothelial Growth Factor AVascular Endothelial Growth Factors