In vivo (Athens, Greece) · 2010
Transplanted mesenchymal stem cells can inhibit the three developmental stages of murine acute graft-versus-host disease.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Li Hong, Guo Zikuan, Zhu Heng, Li Xiu Sen, Jiang Xiaoxia, Yao Huiyu, Wang Xiaoyan, Liao Li, Liu Yuanlin, Wu Ying, Zhang Yi, Mao Ning
Аннотация
BACKGROUND: Recent data have demonstrated that mesenchymal stem cells (MSCs) have potent immune regulation capacity in vitro, enhancing their therapeutic appeal for their utilisation in the management of acute graft-versus-host disease (aGvHD). However, their immunoregulatory activity in vivo is largely unknown.
MATERIALS AND METHODS: Using murine compact bone-derived MSCs in an aGvHD model, the phenotypic status of splenocytes of aGvHD and aGvHD+MSC groups of mice were examined by flow cytometry.
RESULTS: MSC infusion decreased the expression of MHC-II and CD69 molecules on splenic CD11b+ cells of aGvHD mice, which resulted in decreased maturation of antigen-presenting cells. Moreover, the down-regulated ratio of CD3+CD69+ to CD3+ cells, leading to restrained early activation and effector T-cell formation, resulted in the enhancement of the absolute and relative number of splenic CD3+ cells by MSCs co-transfer.
CONCLUSION: This study demonstrated that MSCs can inhibit the three developmental stages of aGvHD.
MATERIALS AND METHODS: Using murine compact bone-derived MSCs in an aGvHD model, the phenotypic status of splenocytes of aGvHD and aGvHD+MSC groups of mice were examined by flow cytometry.
RESULTS: MSC infusion decreased the expression of MHC-II and CD69 molecules on splenic CD11b+ cells of aGvHD mice, which resulted in decreased maturation of antigen-presenting cells. Moreover, the down-regulated ratio of CD3+CD69+ to CD3+ cells, leading to restrained early activation and effector T-cell formation, resulted in the enhancement of the absolute and relative number of splenic CD3+ cells by MSCs co-transfer.
CONCLUSION: This study demonstrated that MSCs can inhibit the three developmental stages of aGvHD.
Предметные рубрики MeSH
Acute DiseaseAnimalsAntigens, CDAntigens, Differentiation, T-LymphocyteCD11b AntigenCD3 ComplexCells, CulturedDisease Models, AnimalFemaleFlow CytometryGraft vs Host DiseaseImmunophenotypingLectins, C-TypeMesenchymal Stem Cell TransplantationMesenchymal Stem CellsMiceMice, Inbred BALB CMice, Inbred C57BLSpleenT-LymphocytesCD69 Antigens