Microbiome of the Proximal Small Intestine in Patients with Acute Pancreatitis.

Currently, due to the complexity of obtaining samples, specific features of laboratory processing and analysis of the results, there is a lack of data on the microbial signature of the small intestine in healthy and diseased states of the upper gastrointestinal tract.

Objective

To investigate the characteristics of the small intestinal microbiome in acute pancreatitis of varying severity and to identify correlations with clinical factors.

Methods

This study included 30 patients with acute pancreatitis of varying severity treated between 1 January 2019 and 31 December 2021. The composition of the microbiota was analyzed by metagenomic sequencing of the 16S rRNA gene from jejunal samples.

Results

The mortality rate in the study group was 23.3%. The small intestinal microbiome was dominated by Streptococcus (median relative abundance 19.2%, interquartile range 6.4-35.1%), Veillonella (3.4%; 0.6-7%), Granulicatella (2.7%; 0.6-5%), Fusobacterium (2.2%; 0.3-5.9%), Prevotella (1.5%; 0.3-8%), Haemophilus (0.9%; 0.2-10%), Gemella (0.8%; 0.2-4.3%), and Lactobacillus (0.2%; 0.1-0.9%). More severe disease was associated with decreased abundance of Neisseria mucosa, Parvimonas micra, and Megasphaera micronuciformis. In contrast, the relative abundance of the genera Streptococcus (species S. rubneri/parasanguinis/australis), Actinomyces, and several genera within the family Enterobacteriaceae was higher in these patients.

Conclusions

The state of the microbiota has important prognostic value and correlates with the duration from the onset of the pain syndrome to the time of receiving qualified care in the hospital.