Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2011
The contrasting effect of estrogen on mRNA expression of VEGF in bovine retinal vascular endothelial cells under different oxygen conditions.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Ma Xiaohua, Bi Hongsheng, Qu Yi, Xie Xiaofeng, Li Jinghai
Аннотация
OBJECTIVE: To investigate the influence of Estradial (E(2)) at physiological concentration on mRNA expression of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1α(HIF-α1) in bovine retinal vascular endothelial cells (BRECs) under different oxygen conditions.
METHODS: The mRNA expression of VEGF, HIF-1α in BRECs was studied by quantitative reverse-transcription PCR (qRT-PCR), and protein levels were assayed by Western Blot. Different concentrations of E(2) (10(-12), 10(-10), 10(-8) mol/l) and estrogen receptor antagonist Tamoxifen (10(-7) mol/l) were added into the cell culture medium of different groups, while the phosphate-buffered saline (PBS) was added in the control group instead. Culture conditions were set to be under normoxia and hypoxia. The results of each group were detected after 8 hours and 24 hours.
RESULTS: (1) Under hypoxia, gene expression of VEGF, HIF-1α in BRECs increased more than that of the control group (P < 0.05). There is evident positive correlation between the mRNA and protein levels of VEGF and HIF-1α (r = 0.82). (2) Treated with 10(-8) mol/l E(2,) the expression of VEGF mRNA was increased in a time-dependent manner (P < 0.05). Treated with the indicated to concentrations of E(2) (10(-12 ∼ -8) mol/l) under normoxia for twenty-four hours, the mRNA expression of VEGF in BRECs was increased in a dose-dependent manner (P < 0.05), whereas E(2) did not influence the mRNA expression of HIF-1α (P > 0.05). (3) Under hypoxia , E(2) reduced the mRNA and protein levels of HIF-1α and VEGF in a concentration-dependent manner (P < 0.05), and the decrease developed in a time-dependent manner (P < 0.05). (4) An overdose of tamoxifen (10(-7) mol/l) reversed the effect of E(2) (10(-8) mol/l) (P < 0.05).
CONCLUSIONS: E(2) increases the gene expression of VEGF in BRECs under normoxia, whereas E(2) reduces the gene expression of VEGF in BRECs through HIF-1α under hypoxic conditions in a dose-dependent and time-dependent manner. The contrasting effect of E(2) on mRNA expression of VEGF may play a prophylactic role in retinopathy of prematurity (ROP).
METHODS: The mRNA expression of VEGF, HIF-1α in BRECs was studied by quantitative reverse-transcription PCR (qRT-PCR), and protein levels were assayed by Western Blot. Different concentrations of E(2) (10(-12), 10(-10), 10(-8) mol/l) and estrogen receptor antagonist Tamoxifen (10(-7) mol/l) were added into the cell culture medium of different groups, while the phosphate-buffered saline (PBS) was added in the control group instead. Culture conditions were set to be under normoxia and hypoxia. The results of each group were detected after 8 hours and 24 hours.
RESULTS: (1) Under hypoxia, gene expression of VEGF, HIF-1α in BRECs increased more than that of the control group (P < 0.05). There is evident positive correlation between the mRNA and protein levels of VEGF and HIF-1α (r = 0.82). (2) Treated with 10(-8) mol/l E(2,) the expression of VEGF mRNA was increased in a time-dependent manner (P < 0.05). Treated with the indicated to concentrations of E(2) (10(-12 ∼ -8) mol/l) under normoxia for twenty-four hours, the mRNA expression of VEGF in BRECs was increased in a dose-dependent manner (P < 0.05), whereas E(2) did not influence the mRNA expression of HIF-1α (P > 0.05). (3) Under hypoxia , E(2) reduced the mRNA and protein levels of HIF-1α and VEGF in a concentration-dependent manner (P < 0.05), and the decrease developed in a time-dependent manner (P < 0.05). (4) An overdose of tamoxifen (10(-7) mol/l) reversed the effect of E(2) (10(-8) mol/l) (P < 0.05).
CONCLUSIONS: E(2) increases the gene expression of VEGF in BRECs under normoxia, whereas E(2) reduces the gene expression of VEGF in BRECs through HIF-1α under hypoxic conditions in a dose-dependent and time-dependent manner. The contrasting effect of E(2) on mRNA expression of VEGF may play a prophylactic role in retinopathy of prematurity (ROP).
Предметные рубрики MeSH
AnimalsBlotting, WesternCattleCells, CulturedDose-Response Relationship, DrugEndothelium, VascularEstradiolEstrogen AntagonistsGene Expression RegulationHypoxiaHypoxia-Inducible Factor 1, alpha SubunitOxygenRNA, MessengerRetinal VesselsReverse Transcriptase Polymerase Chain ReactionTamoxifenTime FactorsVascular Endothelial Growth Factor A