[Zhonghua yan ke za zhi] Chinese journal of ophthalmology · 2009
[Analysis of antibody profiles against retinal antigens in serum from patients with glaucoma through mass spectrometry].
Аннотация на английскомСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Yan Liang, Joachim S C, Pfeiffer N, Grus H F, Erb C
Аннотация
OBJECTIVE: To compare the difference of the antibody profile between the primary open angle glaucoma (POAG) and the healthy controls (CTRL).
METHODS: It was a case-control study. Sera from 44 POAG and 48 CTRL were added to tubes containing pre-washed Protein G beads, which can capture IgGs. These IgG-Protein G-complexes were used to capture target antigens (homogenized bovine retina). After elution, the antigenic proteins were measured through surface-enhanced laser desorption/ionization time-of-flight mass spectrometry. By t-test and multivariate statistical analysis, the spectra, the age and the gender between the two groups were compared; subsequently, the antigenic mass spectra data combined with the type of protein chips and the energy of laser desorption/ionization between these two groups were also compared, which indirectly reflect the antibody profiles of these two groups.
RESULTS: The mean intensity of spectra were 4244.427 +/- 4721.115 in CTRL and 4049.864 +/- 4083.819 in POAG, which showed statistical difference (F = 1.340, P < 0.01); complex antibody profiles were found in both groups. Significantly different immunoreactive spectra were detected between POAG patients and control subjects. Some up-, but mainly down-regulated immunoreactivities were present in POAG patients.
CONCLUSIONS: A significant different pattern of antibody profile is present between the POAG and CTRL. Down-regulated immunoreactivities present in POAG might mean an association between the "aberrant autoimmunity" and the primary open angle glaucoma.
METHODS: It was a case-control study. Sera from 44 POAG and 48 CTRL were added to tubes containing pre-washed Protein G beads, which can capture IgGs. These IgG-Protein G-complexes were used to capture target antigens (homogenized bovine retina). After elution, the antigenic proteins were measured through surface-enhanced laser desorption/ionization time-of-flight mass spectrometry. By t-test and multivariate statistical analysis, the spectra, the age and the gender between the two groups were compared; subsequently, the antigenic mass spectra data combined with the type of protein chips and the energy of laser desorption/ionization between these two groups were also compared, which indirectly reflect the antibody profiles of these two groups.
RESULTS: The mean intensity of spectra were 4244.427 +/- 4721.115 in CTRL and 4049.864 +/- 4083.819 in POAG, which showed statistical difference (F = 1.340, P < 0.01); complex antibody profiles were found in both groups. Significantly different immunoreactive spectra were detected between POAG patients and control subjects. Some up-, but mainly down-regulated immunoreactivities were present in POAG patients.
CONCLUSIONS: A significant different pattern of antibody profile is present between the POAG and CTRL. Down-regulated immunoreactivities present in POAG might mean an association between the "aberrant autoimmunity" and the primary open angle glaucoma.