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Войти в исследования Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology · 2001
[Determination of the point of gene mutation in two types of tissue from the aminoglycoside antibiotics induced deaf patients].
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Xu L, Chen W, Xing C
Аннотация
OBJECTIVE: To elucidate the gene mutation presents in hair follicle cells, just as blood cells, in the aminoglycoside antibiotics induced deaf patients(AAID).
METHOD: Mitochondrial DNA of blood cells and/or hair follicle cells from 8 members of 3 aminoglycoside antibiotics induced deafness families were analysed using PCR-SSCP and Alw 26 I digestian.
RESULT: Our result showed that a gene mutation at nucleotide 1555 in 12 S rRNA of mitochondrial DNA appeared in blood cells and/or hair follicle cells of 7 subjects among those 8 members. Only a father with normal hearing did't exhibit such a mutation.
CONCLUSION: This indicated that hairs could be used as a sample instead of blood to perform gene examination for AAID.
METHOD: Mitochondrial DNA of blood cells and/or hair follicle cells from 8 members of 3 aminoglycoside antibiotics induced deafness families were analysed using PCR-SSCP and Alw 26 I digestian.
RESULT: Our result showed that a gene mutation at nucleotide 1555 in 12 S rRNA of mitochondrial DNA appeared in blood cells and/or hair follicle cells of 7 subjects among those 8 members. Only a father with normal hearing did't exhibit such a mutation.
CONCLUSION: This indicated that hairs could be used as a sample instead of blood to perform gene examination for AAID.