Electrolyte composition of tears in normal dogs and its comparison to serum and plasma.

PURPOSE: The dog is an important animal model for tear dysfunction diseases, however to-date the electrolyte composition of the dog's tears is unknown. The aim of this study was to analyze the electrolyte content of canine tears and compare it to serum and plasma.

METHODS

Tear samples were collected from 18 eyes of 9 dogs. Blood for serum was collected in tubes with no anticoagulants; plasma was obtained by using two different anticoagulants: Citrate-Phosphate-Dextrose (CPD) and heparin. The electrolytes were measured in all samples, analyzed, and compared.

RESULTS

Most of the electrolyte values in tears were statistically different (P < 0.05) from electrolyte values in serum and plasma. Potassium and chloride values were significantly higher in tears compared to serum and plasma, while calcium and phosphate values were significantly lower. Sodium values in tears were higher than in serum and heparinized-plasma, but lower than CPD-plasma. Bicarbonate values were lower in tears compared to serum and heparinized plasma, but was not statistically different than CPD-plasma. While magnesium values were lower in tears compared to serum and heparinized-plasma, the difference was not statistically different.

CONCLUSIONS

Herein, we report for the first time the electrolyte composition of the canine tears and its comparison to serum and plasma.