Pathogenesis of swine influenza virus subtype H1N2 infection in pigs.

The purpose of this study was to elucidate pathogenesis and viral distribution in pigs infected with swine influenza virus subtype H1N2, over a period of 10 days, by morphometric analysis and in-situ hybridization. Fifteen colostrum-deprived pigs aged 3 weeks were inoculated intranasally with virus. Pneumonia was severe at 1 day post-inoculation (dpi), moderate at 3 and 5 dpi, and mild at 7 and 10 dpi. The pulmonary lesion score was correlated with the score of cells positive by in-situ hybridization for swine influenza virus (r(s)= 0.9114, P< 0.05). The distribution of swine influenza virus varied according to the duration of infection. At 1 and 3 dpi, hybridization signals were detected mainly in the bronchial and bronchiolar epithelial cells, but they were detected mainly in the pneumocytes and macrophages (alveolar and interstitial) at 7 and 10 dpi. The results confirmed that swine influenza virus subtype H1N2, isolated in Korea, is a virulent pathogen causing severe pneumonia.