International journal of antimicrobial agents · 2025
Molecular epidemiology and genomic insights into Enterobacter cloacae complex co-harbouring blaKPC and mcr: Implications for resistance gene transmission.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Wang Boqian, Fu Jingjing, He Yaqing, Li Kexin, Hu Guifang, Hu Mingda, Wang Xin, Liu Wanqiu, Zhang Rui, Wang Yuxin, Aimaiti Buaijier, Zhao Xinru, Song Hongbin, Hu Xiaofeng, Ren Hongguang
Аннотация
OBJECTIVE: This study aims to systematically investigate the molecular epidemiology and genomic characteristics of Enterobacter cloacae complex (ECC) strains globally harbouring blaKPC and mcr, as well as the co-existence of drug resistance genes. The goal is to provide insights and recommendations for monitoring clinical drug-resistant strains and super-resistant plasmids.
METHODS: This study analysed 281 ECC isolates harbouring both blaKPC and mcr, obtained from NCBI GenBank database (2003-2024) with whole-genome sequencing data. We constructed a phylogenetic tree of ECC strains for phylogenetic analysis. Resistance genes were identified using the ABRicate and CARD databases, and their distribution was examined. Plasmid replicon types for blaKPC and mcr were analysed with PlasmidFinder, including upstream and downstream genetic environments of these genes.
RESULTS: The predominant genotype combinations harbouring both blaKPC and mcr are blaKPC-3 and mcr-9.1, followed by blaKPC-4 and mcr-9.1, with the dominant strain being E. hormaechei ssp. xiangfangensis. The IncHI2(2A) plasmid co-harbouring blaKPC and mcr-9 was mainly detected in genomes from the United States. Phylogenetic analysis indicated that the blaKPC-mcr-9-IncHI2(2A) plasmids in ECC strains have a high genetic similarity to mcr-9-IncHI2(2A), suggesting that the latter may have acquired the additional highly transferable blaKPC.
CONCLUSIONS: ECC strains have become an important reservoir cluster for blaKPC and mcr-9, and the IncHI2(2A) plasmid is a potential vector for the horizontal co-transmission of carbapenem and colistin resistance genes. Effective monitoring should be implemented to assess the prevalence of co-harbouring blaKPC and mcr-9 in individual ECC isolates and even in single plasmid.
METHODS: This study analysed 281 ECC isolates harbouring both blaKPC and mcr, obtained from NCBI GenBank database (2003-2024) with whole-genome sequencing data. We constructed a phylogenetic tree of ECC strains for phylogenetic analysis. Resistance genes were identified using the ABRicate and CARD databases, and their distribution was examined. Plasmid replicon types for blaKPC and mcr were analysed with PlasmidFinder, including upstream and downstream genetic environments of these genes.
RESULTS: The predominant genotype combinations harbouring both blaKPC and mcr are blaKPC-3 and mcr-9.1, followed by blaKPC-4 and mcr-9.1, with the dominant strain being E. hormaechei ssp. xiangfangensis. The IncHI2(2A) plasmid co-harbouring blaKPC and mcr-9 was mainly detected in genomes from the United States. Phylogenetic analysis indicated that the blaKPC-mcr-9-IncHI2(2A) plasmids in ECC strains have a high genetic similarity to mcr-9-IncHI2(2A), suggesting that the latter may have acquired the additional highly transferable blaKPC.
CONCLUSIONS: ECC strains have become an important reservoir cluster for blaKPC and mcr-9, and the IncHI2(2A) plasmid is a potential vector for the horizontal co-transmission of carbapenem and colistin resistance genes. Effective monitoring should be implemented to assess the prevalence of co-harbouring blaKPC and mcr-9 in individual ECC isolates and even in single plasmid.