Archives of oral biology · 2026
Enterococcus faecalis promotes orthodontic tooth movement in mice by M1-like macrophage polarization.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
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Ошибки и ограничения
История · было → стало
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Yu Fei, Jiang Ting, Lu Wenxin, Peng Jiajia, Ouyang Ningjuan, Zhao Zhihe
Аннотация
OBJECTIVE: Probiotic-mediated therapy has potential role in influencing bone remodeling. In this study, we aim to explore the impact of microorganisms on accelerating orthodontic tooth movement (OTM).
DESIGN: Potential probiotic candidates were identified through 16S rRNA gene sequencing in a mouse model of OTM. To investigate the osteoimmunomodulatory impact, primary mouse periodontal ligament cells (PDLCs) were cultured with or without conditioned media (CM) derived from macrophages post-incubation with microorganisms. The microbiota of the mice has be depleted using broad-spectrum antibiotic mixture (ABX) before being administered with microorganisms. The distance of OTM have been measured, and the alveolar bone have been analyzed using micro-CT and immunohistochemical staining.
RESULTS: The relative abundance of Enterococcus faecalis (E. faecalis) increased in the move group. The results demonstrated that E. faecalis increased the number of M1-polarized macrophages, and a decreased osteogenic level in PDLCs treated with CM E. faecalis group. The distance of OTM in ABX mice has been increased via administration of E. faecalis.
CONCLUSIONS: This study has explored the potential effects of E. faecalis administration on the OTM process through immunomodulation. By elucidating this mechanism, our work lays the groundwork for developing future microorganism-interventions that could safely accelerate OTM with non-invasive strategy.
DESIGN: Potential probiotic candidates were identified through 16S rRNA gene sequencing in a mouse model of OTM. To investigate the osteoimmunomodulatory impact, primary mouse periodontal ligament cells (PDLCs) were cultured with or without conditioned media (CM) derived from macrophages post-incubation with microorganisms. The microbiota of the mice has be depleted using broad-spectrum antibiotic mixture (ABX) before being administered with microorganisms. The distance of OTM have been measured, and the alveolar bone have been analyzed using micro-CT and immunohistochemical staining.
RESULTS: The relative abundance of Enterococcus faecalis (E. faecalis) increased in the move group. The results demonstrated that E. faecalis increased the number of M1-polarized macrophages, and a decreased osteogenic level in PDLCs treated with CM E. faecalis group. The distance of OTM in ABX mice has been increased via administration of E. faecalis.
CONCLUSIONS: This study has explored the potential effects of E. faecalis administration on the OTM process through immunomodulation. By elucidating this mechanism, our work lays the groundwork for developing future microorganism-interventions that could safely accelerate OTM with non-invasive strategy.