Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2013
[The study of interfering of endogenous VEGF-C genes and the protein expression gastric cancer cell with siRNA technique].
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Guo Mei-Mei, Wang Yong, Han Fang-Hai
Аннотация
OBJECTIVE: To explore the interfering effects of siRNA on endogenous VEGF-C genes and the protein expression in gastric cancer cells.
METHODS: Cultured gastric cancer cell line SGC7901 cells were prepared in vitro. Five siRNA primers of VEGF-C were designed depending on the sequence of Accession No. BC035212. 1 in Genbank. After homology analysis, the primers were synthesized and transfected into SGC7901 cells. The endogenous VEGF-C mRNA level and its protein expression were observed.
RESULTS: VEGF-C-siRNA was inserted into the gastric cancer cell successfully. Five siRNA primers of VEGF-C could inhibit VEGF-C genes and protein expression.
CONCLUSION: siRNA could block the endogenous VEGF-C genes and the protein expression in gastric cancer cell.
METHODS: Cultured gastric cancer cell line SGC7901 cells were prepared in vitro. Five siRNA primers of VEGF-C were designed depending on the sequence of Accession No. BC035212. 1 in Genbank. After homology analysis, the primers were synthesized and transfected into SGC7901 cells. The endogenous VEGF-C mRNA level and its protein expression were observed.
RESULTS: VEGF-C-siRNA was inserted into the gastric cancer cell successfully. Five siRNA primers of VEGF-C could inhibit VEGF-C genes and protein expression.
CONCLUSION: siRNA could block the endogenous VEGF-C genes and the protein expression in gastric cancer cell.