[The study of interfering of endogenous VEGF-C genes and the protein expression gastric cancer cell with siRNA technique].

OBJECTIVE

To explore the interfering effects of siRNA on endogenous VEGF-C genes and the protein expression in gastric cancer cells.

METHODS

Cultured gastric cancer cell line SGC7901 cells were prepared in vitro. Five siRNA primers of VEGF-C were designed depending on the sequence of Accession No. BC035212. 1 in Genbank. After homology analysis, the primers were synthesized and transfected into SGC7901 cells. The endogenous VEGF-C mRNA level and its protein expression were observed.

RESULTS

VEGF-C-siRNA was inserted into the gastric cancer cell successfully. Five siRNA primers of VEGF-C could inhibit VEGF-C genes and protein expression.

CONCLUSION

siRNA could block the endogenous VEGF-C genes and the protein expression in gastric cancer cell.