Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology · 2008
[Construction of helper plasmids used in reverse genetic system of a cell adapted rabies virus strain].
Оценочное исследованиеСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Huang Ying, Hu Rong-Liang, Tang Qing
Аннотация
OBJECTIVE: To construct four helper plasmids which are necessary in reverse genetic system of a cell adapted rabies virus strain.
METHODS: Nucleoprotein gene, phosphprotein gene, glycoprotein gene and the viral RNA polymerse gene are amplified by RT-PCR. The four gene fragments were cloned into an expression vector respectively after sequencing to construct four helper plasmids.
RESULTS: The four structral genus were amplified successfully, the sequence were correct. And all the structral protein gene fragments were cloned into expression vectors, the results were correct identified by cleavage reaction.
CONCLUSION: Construction of the four helper plasmids have been done successfully, and it is the basis for rescuing the adapted cell rabies virus strain from cloned cDNA.
METHODS: Nucleoprotein gene, phosphprotein gene, glycoprotein gene and the viral RNA polymerse gene are amplified by RT-PCR. The four gene fragments were cloned into an expression vector respectively after sequencing to construct four helper plasmids.
RESULTS: The four structral genus were amplified successfully, the sequence were correct. And all the structral protein gene fragments were cloned into expression vectors, the results were correct identified by cleavage reaction.
CONCLUSION: Construction of the four helper plasmids have been done successfully, and it is the basis for rescuing the adapted cell rabies virus strain from cloned cDNA.