[Construction of helper plasmids used in reverse genetic system of a cell adapted rabies virus strain].

OBJECTIVE

To construct four helper plasmids which are necessary in reverse genetic system of a cell adapted rabies virus strain.

METHODS

Nucleoprotein gene, phosphprotein gene, glycoprotein gene and the viral RNA polymerse gene are amplified by RT-PCR. The four gene fragments were cloned into an expression vector respectively after sequencing to construct four helper plasmids.

RESULTS

The four structral genus were amplified successfully, the sequence were correct. And all the structral protein gene fragments were cloned into expression vectors, the results were correct identified by cleavage reaction.

CONCLUSION

Construction of the four helper plasmids have been done successfully, and it is the basis for rescuing the adapted cell rabies virus strain from cloned cDNA.