Laryngoscope investigative otolaryngology · 2025
Efficacy of Topical Nigella sativa Oil for Oral Wound Healing in Rats.
СтатьяНазвание и аннотация: Перевод в очереди
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Tabaru Alper, Rüzgar Sedat
Аннотация
OBJECTIVE: This study aimed to examine the effect of topical Nigella sativa oil (NSO) on intraoral wound healing in rats.
MATERIALS AND METHODS: Forty-eight female Wistar albino rats (2.5-3 months old) were assigned to two groups (n = 24 each): A control group receiving no treatment and an NSO group receiving a topical application of 1 cc NSO. A standardized circular full-thickness wound (3 mm in diameter) was created on the mucoperiosteum of the hard palate. The animals were sacrificed on postoperative Days 3, 7, 14, and 20 to assess wound area macroscopically, wound diameter histologically, and immunohistochemically for anti-transforming growth factor-β (anti-TGF-β) and anti-vascular growth factor (anti-VGF).
RESULTS: No significant differences were observed between groups on days 3 and 20 (p > 0.05). On Days 7 and 14, however, the NSO group exhibited significantly smaller wound areas and wound diameters (p < 0.05). Immunohistochemical staining showed lower TGF-β expression on Days 7 and 14 in the NSO group compared to control (p < 0.05), whereas VGF staining did not differ significantly (p > 0.05).
CONCLUSION: Topical NSO accelerated the healing of intraoral wounds, particularly by enhancing re-epithelialization between postoperative days 7 and 14. This effect appears to be mediated through TGF-β modulation and NSO's known anti-inflammatory, antimicrobial, and antioxidant actions. Topical NSO may thus be considered as an adjunct to conventional wound management in the oral cavity.
LEVEL OF EVIDENCE: NA.
MATERIALS AND METHODS: Forty-eight female Wistar albino rats (2.5-3 months old) were assigned to two groups (n = 24 each): A control group receiving no treatment and an NSO group receiving a topical application of 1 cc NSO. A standardized circular full-thickness wound (3 mm in diameter) was created on the mucoperiosteum of the hard palate. The animals were sacrificed on postoperative Days 3, 7, 14, and 20 to assess wound area macroscopically, wound diameter histologically, and immunohistochemically for anti-transforming growth factor-β (anti-TGF-β) and anti-vascular growth factor (anti-VGF).
RESULTS: No significant differences were observed between groups on days 3 and 20 (p > 0.05). On Days 7 and 14, however, the NSO group exhibited significantly smaller wound areas and wound diameters (p < 0.05). Immunohistochemical staining showed lower TGF-β expression on Days 7 and 14 in the NSO group compared to control (p < 0.05), whereas VGF staining did not differ significantly (p > 0.05).
CONCLUSION: Topical NSO accelerated the healing of intraoral wounds, particularly by enhancing re-epithelialization between postoperative days 7 and 14. This effect appears to be mediated through TGF-β modulation and NSO's known anti-inflammatory, antimicrobial, and antioxidant actions. Topical NSO may thus be considered as an adjunct to conventional wound management in the oral cavity.
LEVEL OF EVIDENCE: NA.