Annales d'endocrinologie · 2023
Diagnostic performance of an automated immunoassay for salivary cortisol.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
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Laguillier-Morizot Christelle, Bonnet-Serrano Fidéline, Leguy Marie-Clémence, Simeonovic Miliça, Sée Catherine, Zientek Corinne, Soussan Mickael, Bouys Lucas, Bertherat Jérôme, Guibourdenche Jean
Аннотация
Automated immunoanalysis (AI) is an interesting alternative for measuring salivary cortisol, as the gold standard HPLC-MS/MS method is not yet readily available. The aim of this study was to evaluate the diagnostic performance of salivary cortisol immunoassay on the iSYS immunoanalyzer in adrenal dynamic tests. Cortisol was measured on iSYS and on HPLC-MS/MS in saliva samples collected after 1mg-dexamethasone suppression test (DST) in 115 patients suspected of Cushing syndrome, and during Synacthen® stimulation test (SST) in 108 patients suspected of adrenal insufficiency. Concentrations on AI correlated well with HPLC-MS/MS (Spearman r=0.9496; P<0.0001), but with a significant positive bias. ROC analysis of salivary cortisol identified optimal cut-off values on AI and HPLC-MS/MS of respectively 3.5 and 0.77nmol/L for DST and 32.6 and 13.8nmol/L at T60 after SST. Automated immunoassays for salivary cortisol are suitable in daily practice but require determination of specific cut-off and reference values.