Zhonghua fu chan ke za zhi · 2012
[Effect of follicle stimulating hormone on the secretion of anti-mullerian hormone in the granulosa cells in patients with polycystic ovarian syndrome].
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Li Yi, Liang Xiao-yan
Аннотация
OBJECTIVE: To study the effect of follicle stimulating hormone (FSH) on the secretion of anti-mullerian hormone (AMH) in granulosa cells in patients with polycystic ovarian syndrome (PCOS).
METHODS: From Aug. 2008 to Dec. 2009, 33 patients with PCOS in Reproductive Medicine Research Center, Sixth Affiliated Hospital of Sun Yat-sen University were collected from 8-10 mm antral follicles and classified into the following three groups: FSH-unstimulated granulosa cells (unstimulated group, n = 12), exogenous FSH-stimulated granulosa cells (exo-stimulated group, n = 12) and internal FSH-stimulated granulosa cells (int-stimulated group, n = 21). ELISA and real-time PCR were relatively used to measure protein and mRNA level of AMH. Luciferase reporting vector was constructed to detect the promoter activity of AMH.
RESULTS: The levels of AMH secretion in PCOS granulosa cells were (11.4 ± 4.0) µg/L in unstimulated group, (7.9 ± 1.1) µg/L in exo-stimulated group and (5.6 ± 1.7) µg/L in int-stimulated group. Both the external and internal stimulation of FSH may suppress AMH secretion significantly (P < 0.05). The mRNA level of AMH in PCOS granulosa cells were 2.5 ± 1.2 in unstimulated group, which were higher than 1.5 ± 0.5 in exo-stimulated group and 1.1 ± 0.7 in int-stimulated group significantly (P < 0.05). The luciferase activity of AMH in PCOS granulosa cells were 11.5 ± 2.3 in unstimulated group, 8.7 ± 2.4 in exo-stimulated group and 6.8 ± 2.4 in int-stimulated group. The luciferase activity of AMH in unstimulated group was significantly higher than those in exo-stimulated and int-stimulated groups (P < 0.05).
CONCLUSION: FSH may inhibit the excessive secretion of AMH and stimulate follicle growth in PCOS granulosa cells by suppressing activity and expression of promoter.
METHODS: From Aug. 2008 to Dec. 2009, 33 patients with PCOS in Reproductive Medicine Research Center, Sixth Affiliated Hospital of Sun Yat-sen University were collected from 8-10 mm antral follicles and classified into the following three groups: FSH-unstimulated granulosa cells (unstimulated group, n = 12), exogenous FSH-stimulated granulosa cells (exo-stimulated group, n = 12) and internal FSH-stimulated granulosa cells (int-stimulated group, n = 21). ELISA and real-time PCR were relatively used to measure protein and mRNA level of AMH. Luciferase reporting vector was constructed to detect the promoter activity of AMH.
RESULTS: The levels of AMH secretion in PCOS granulosa cells were (11.4 ± 4.0) µg/L in unstimulated group, (7.9 ± 1.1) µg/L in exo-stimulated group and (5.6 ± 1.7) µg/L in int-stimulated group. Both the external and internal stimulation of FSH may suppress AMH secretion significantly (P < 0.05). The mRNA level of AMH in PCOS granulosa cells were 2.5 ± 1.2 in unstimulated group, which were higher than 1.5 ± 0.5 in exo-stimulated group and 1.1 ± 0.7 in int-stimulated group significantly (P < 0.05). The luciferase activity of AMH in PCOS granulosa cells were 11.5 ± 2.3 in unstimulated group, 8.7 ± 2.4 in exo-stimulated group and 6.8 ± 2.4 in int-stimulated group. The luciferase activity of AMH in unstimulated group was significantly higher than those in exo-stimulated and int-stimulated groups (P < 0.05).
CONCLUSION: FSH may inhibit the excessive secretion of AMH and stimulate follicle growth in PCOS granulosa cells by suppressing activity and expression of promoter.