Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria · 2021
Sarcocystidae in wild birds of southeastern Brazil.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
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Rêgo Wagner Martins Fontes do, Costa Júlia Gatti Ladeia, Baraviera Ramon Castro de Araujo, Pinto Lorena Velozo, Bessa Gabriella de Lima, Lopes Rosálida Estevam Nazar, Silveira Julia Angelica Gonçalves da, Vitor Ricardo Wagner Almeida
Аннотация
This study aimed to identify members of the Sarcocystidae family in naturally infected wild birds at a rescue center in the state of Minas Gerais, southeastern Brazil. The heart and brain of 44 wild birds were evaluated by bioassay in mice to detect T. gondii, and extracted DNA was used for nested PCR of the 18S ribosomal DNA gene to detect members of the Sarcocystidae family. The positive samples were sequenced, assembled, edited and compared with sequences deposited in GenBank. Toxoplasma gondii was isolated from six (13.6%) out of 44 birds. Toxoplasma gondii DNA was identified in 10/44 (22.7%) of the birds. The amplified sequences exhibited 100% similarity with the DNA of the ME49 strain of T. gondii. Sarcocystis DNA (99% similarity) was identified in 5/44 (11.4%) of the birds. T. gondii and Sarcocystis spp. are common in wild birds in Minas Gerais, Brazil.