Frontiers in microbiology · 2025
Sub-inhibitory concentrations of oxacillin modulate biogenesis and function of extracellular vesicles secreted by oxacillin-sensitive methicillin-resistant Staphylococcus aureus.
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Ran Zhaoxia, Hao Minghui, Guo Binxin, Wang Junrui
Аннотация
BACKGROUND: Extracellular vesicles (EVs) derived from Staphylococcus aureus (S. aureus) carry multiple components, such as toxins, antigens, and resistance determinants, whose production is influenced by exposure to β-lactam antibiotics. However, systematic studies on the effects of β-lactam antibiotics on the release and functions of EVs remain limited.
METHODS: Extracellular vesicles (EVs) were isolated from the OS-MRSA (OS-200) strain by ultracentrifugation, including both EVs untreated with antibiotics and those exposed to sub-inhibitory concentrations of oxacillin (OI-EVs). Proteomic analysis was performed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The functional changes of these EVs were further assessed through erythrocyte hemolysis assays, biofilm formation assays, measurement of cytokines, and cell invasion assays.
RESULTS: Exposure to sub-inhibitory concentrations of oxacillin (half and one-eighth of the minimum inhibitory concentration [MIC]) significantly enhanced the secretion of OI-EVs and increased the abundance of several proteins, including penicillin-binding protein 2, EmrB, Hlg, and enolase, in a concentration-dependent manner. Notably, EV1/2MIC exhibited more pronounced functional changes and enhanced hemolytic activity against rabbit red blood cells. Also, EV1/2MIC exhibited significant promoting effects on biofilm formation of OS-200. Additionally, OI-EVs stimulated the secretion of interleukin-6 and tumor necrosis factor-α by THP-1 macrophages in a dose-dependent manner and demonstrated greater penetration potential into A549 lung epithelial cells.
CONCLUSION: Exposure to sub-inhibitory concentrations of oxacillin significantly altered the secretion and composition of EVs, highlighting a novel relationship between antimicrobial exposure, exosome biogenesis, and OS-MRSA pathogenicity. These findings provide new insights into how β-lactam antibiotics influence host-pathogen interactions by modulating the secretion of bacterial vesicles.
METHODS: Extracellular vesicles (EVs) were isolated from the OS-MRSA (OS-200) strain by ultracentrifugation, including both EVs untreated with antibiotics and those exposed to sub-inhibitory concentrations of oxacillin (OI-EVs). Proteomic analysis was performed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The functional changes of these EVs were further assessed through erythrocyte hemolysis assays, biofilm formation assays, measurement of cytokines, and cell invasion assays.
RESULTS: Exposure to sub-inhibitory concentrations of oxacillin (half and one-eighth of the minimum inhibitory concentration [MIC]) significantly enhanced the secretion of OI-EVs and increased the abundance of several proteins, including penicillin-binding protein 2, EmrB, Hlg, and enolase, in a concentration-dependent manner. Notably, EV1/2MIC exhibited more pronounced functional changes and enhanced hemolytic activity against rabbit red blood cells. Also, EV1/2MIC exhibited significant promoting effects on biofilm formation of OS-200. Additionally, OI-EVs stimulated the secretion of interleukin-6 and tumor necrosis factor-α by THP-1 macrophages in a dose-dependent manner and demonstrated greater penetration potential into A549 lung epithelial cells.
CONCLUSION: Exposure to sub-inhibitory concentrations of oxacillin significantly altered the secretion and composition of EVs, highlighting a novel relationship between antimicrobial exposure, exosome biogenesis, and OS-MRSA pathogenicity. These findings provide new insights into how β-lactam antibiotics influence host-pathogen interactions by modulating the secretion of bacterial vesicles.