Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases · 2004
[Application of EITB in immunodiagnosis of cysticercosis].
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Wang Li-na, Ge Ling-yun, Miao Feng, Yu Zhen-hua, Liu Yu-bing, Zhen Tian-min, Li Gui-ping, Yang Shu-fang
Аннотация
OBJECTIVE: To establish a sensitive and specific method EITB for the immunodiagnosis of cysticercosis.
METHODS: Two-dimensional electrophoresis was used to analyze and purify the cyst fluid antigen. Western blotting using the sera of patients with cysticercosis, hydatidosis, and other heteroserum was used to screen the specific antigens. EITB using the specific antigen was established and compared with ELISA. The detection effect of serum and blood on filter paper in EITB was also compared.
RESULTS: Two specific antigens with isoelectric point (pI) of 9.4 and Mr of 14000 and 16600 were obtained, respectively. EITB method based on these antigens was established with the sensitivity and specificity of 92.5% and 100%, respectively, significantly higher than that of ELISA. The sensitivity of serum and filter paper blood was similar in EITB.
CONCLUSION: A sensitive and specific EITB method for immunodiagnosis of cysticercosis was established.
METHODS: Two-dimensional electrophoresis was used to analyze and purify the cyst fluid antigen. Western blotting using the sera of patients with cysticercosis, hydatidosis, and other heteroserum was used to screen the specific antigens. EITB using the specific antigen was established and compared with ELISA. The detection effect of serum and blood on filter paper in EITB was also compared.
RESULTS: Two specific antigens with isoelectric point (pI) of 9.4 and Mr of 14000 and 16600 were obtained, respectively. EITB method based on these antigens was established with the sensitivity and specificity of 92.5% and 100%, respectively, significantly higher than that of ELISA. The sensitivity of serum and filter paper blood was similar in EITB.
CONCLUSION: A sensitive and specific EITB method for immunodiagnosis of cysticercosis was established.