Evaluation of the intestinal transmembrane transport and absorption characteristics of mangiferin in rats by the Ussing chamber technique.

Mangiferin, one of the primary bioactive components in mango, exhibits diverse biological activities but relatively low absorption. Elucidating its absorption characteristics will facilitate the exploitation of its nutritional potential and the optimization of its bioavailability. This study employed liquid chromatography-mass spectrometry to determine mangiferin concentrations in rat plasma and used the Ussing chamber technique to evaluate intestinal absorption of mangiferin at varying concentrations, while comparing absorption differences across distinct intestinal segments. The results demonstrated that after oral administration of 200 mg/kg mangiferin to rats, the peak plasma concentration of 166.80 ng/mL was reached at approximately 2.1 h. The absorption of mangiferin in the ileum, cecum, and proximal colon of rats exhibited a concentration-dependent effect. Compared with the ileum, cecum, and mid-colon, mangiferin demonstrated significantly better absorption in the proximal colon (p < 0.05), indicating that the proximal colon serves as the primary intestinal site for mangiferin absorption into systemic circulation.