Yan ke xue bao = Eye science · 2001
[Effects of angiotensin II on the 3H-TdR incorporation and synthesis of collagen in cultured bovine trabecular meshwork cells].
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Shen F, Zhang L, Liu T
Аннотация
OBJECTIVE: To investigate the effects of angiotensin II (Ang II) on the cell proliferation and collagen synthesis of bovine trabecular meshwork (TM) cells in vitro. It may probe into the mechanism of primary open-angle glaucoma(POAG).
METHODS: (1) The bovine trabecular meshwork cells were cultured and identified by immunohistochemistry method(neuronal specific enolas, NSE, factor related antigan). The growing characteristics and morphological feature of cultured primary and passaged cells were observed by invered microscope and eletron-microscope; (2) Ang II(1 x 10(-7) mol.L-1 and 1 x 10(-8) mol.L-1) and angiotensin receptor type I (AT1) antagonist (losartan) were incubated with cultured TM cells. The cellular proliferation was measured by 3H-thymidine(3H-TdR) incorporation assay and collagen synthesis was indirectly professed though detects the hydroxyproline of medium with chemistry methods.
RESULTS: The cells of bovine trabecular meshwork were cultured successfully. Most of them were epithelia type. Ang II increased the TM cells uptake of 3H-TdR and inhibited by losartan partially. Meanwhile the hydroxyproline of medium was increased correspondingly.
CONCLUSION: Establishing the method of culturing bovine trabecular meshwork cells is an important method for researching the characteristics of TM cells. Ang II can induce the cell proliferation of bovine TM cells and increase the synthesis of collagen in vitro. The AT1 antagonist may inhibit this cell proliferation effect.
METHODS: (1) The bovine trabecular meshwork cells were cultured and identified by immunohistochemistry method(neuronal specific enolas, NSE, factor related antigan). The growing characteristics and morphological feature of cultured primary and passaged cells were observed by invered microscope and eletron-microscope; (2) Ang II(1 x 10(-7) mol.L-1 and 1 x 10(-8) mol.L-1) and angiotensin receptor type I (AT1) antagonist (losartan) were incubated with cultured TM cells. The cellular proliferation was measured by 3H-thymidine(3H-TdR) incorporation assay and collagen synthesis was indirectly professed though detects the hydroxyproline of medium with chemistry methods.
RESULTS: The cells of bovine trabecular meshwork were cultured successfully. Most of them were epithelia type. Ang II increased the TM cells uptake of 3H-TdR and inhibited by losartan partially. Meanwhile the hydroxyproline of medium was increased correspondingly.
CONCLUSION: Establishing the method of culturing bovine trabecular meshwork cells is an important method for researching the characteristics of TM cells. Ang II can induce the cell proliferation of bovine TM cells and increase the synthesis of collagen in vitro. The AT1 antagonist may inhibit this cell proliferation effect.