Physica medica : PM : an international journal devoted to the applications of physics to medicine and biology : official journal of the Italian Association of Biomedical Physics (AIFB) · 2026
Boron neutron capture therapy (BNCT) induces DNA damage on human cholangiocarcinoma cell line.
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Kartini Dea Aulia, Malad Yuwadee, Karoon Pharewa, Kondo Natsuko, Sakurai Yoshinori, Talabnin Krajang, Talabnin Chutima, Kobdaj Chinorat
Аннотация
PURPOSE: Boron neutron capture therapy (BNCT) is a radiation therapy based on the nuclear reaction between 10B and neutrons to induce DNA damage in cancer cells. This study aims to evaluate 10B accumulation in the human cholangiocarcinoma cell line (KKU-055) and measure the cellular response to BNCT.
METHODS: The cytotoxicity of 10B on KKU-055 cell line was observed with different L-BPA fructose concentrations. The amount of 10B in cells was measured via inductively coupled plasma - mass spectrometry. Cells treated with various 10B concentrations were exposed to thermal neutron beams with a flux of 1.89 x 109 n cm-2 s-1 for 5 and 30 min. Survival curve and DNA damage were measured to demonstrate the biological endpoints of cells after irradiation.
RESULTS: The cytotoxicity test revealed that L-BPA fructose up to 51 ppm of 10B is less toxic to the cells with >85% of the control. 10B accumulation in cells was time-dependent, reaching a maximum of 0.126 ppm after 24 h. The number of γH2AX foci was increased in 10B concentration- and irradiation time-dependent manner. A significant decrease in survival fraction was observed after 5 min of BNCT irradiation, with survival fraction dropping to 11.7%.
CONCLUSIONS: These findings showed a high accumulation of 10B in KKU-055 cells could be observed after 24 h. BNCT has proven effective in increasing the radiosensitivity of KKU-055 cell line and enhancing cell death in KKU-055 cells. Therefore, BNCT is a promising technique for cholangiocarcinoma treatment.
METHODS: The cytotoxicity of 10B on KKU-055 cell line was observed with different L-BPA fructose concentrations. The amount of 10B in cells was measured via inductively coupled plasma - mass spectrometry. Cells treated with various 10B concentrations were exposed to thermal neutron beams with a flux of 1.89 x 109 n cm-2 s-1 for 5 and 30 min. Survival curve and DNA damage were measured to demonstrate the biological endpoints of cells after irradiation.
RESULTS: The cytotoxicity test revealed that L-BPA fructose up to 51 ppm of 10B is less toxic to the cells with >85% of the control. 10B accumulation in cells was time-dependent, reaching a maximum of 0.126 ppm after 24 h. The number of γH2AX foci was increased in 10B concentration- and irradiation time-dependent manner. A significant decrease in survival fraction was observed after 5 min of BNCT irradiation, with survival fraction dropping to 11.7%.
CONCLUSIONS: These findings showed a high accumulation of 10B in KKU-055 cells could be observed after 24 h. BNCT has proven effective in increasing the radiosensitivity of KKU-055 cell line and enhancing cell death in KKU-055 cells. Therefore, BNCT is a promising technique for cholangiocarcinoma treatment.