[Effect of recombinant human growth hormone on hypoalbuminemia in peritoneal sepsis: experimental and clinical research].

OBJECTIVE

To investigate the effect of recombinant human growth hormone(rhGH) on hypoalbuminemia in peritoneal sepsis.

METHODS

We observed rhGH on albumin mRNA expression and albumin sythesis in vitro and in vivo with the method of reverse-transcriptional polymerase chain reaction (RT-PCR).

RESULTS

rhGH significantly up-regulated albumin mRNA expression in vitro (143 +/- 6 vs 100.4 +/- 2.2, P < 0.01) and in vivo(152 +/- 8 Vs 100 +/- 3, P < 0.01), and obviously alleviated the inhibition of albumin mRNA expression induced by LPS (P < 0.05). In cecal ligation and puncture-induced septic rats, rhGH significantly raised albumin synthesis(20.4 +/- 1.7 Vs 15.5 +/- 2.9 g/L, P < 0.01) and albumin mRNA expression. In patients with peritoneal sepsis, rhGH in combination with total parenteral nutrition markedly increased serum albumin, prealbumin, and transferrin concentration, but no apparent effect was observed in controls.

CONCLUSION

rhGH significantly increases albumin mRNA expression and albumin synthesis in peritoneal sepsis.