Inhalation toxicology · 2026
PET/CT imaging-based quantification of respiratory tract deposition of small- and large-particle aerosols versus intranasal bolus in rodents.
СтатьяСравнительное исследованиеНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Boydston Jeremy A, Yang Hee-Jeong, Mazur Steve, Bohannon Jordan K, Lackemeyer Matthew G, Byrum Russ, Bartos Christopher, Cooper Kurt, Reeder Rebecca, Kendall William, Conway Kathryn, Partner Kristi, Vaughan Nicholas, Calcagno Claudia, Godwin Gerald, Cong Yu, Crozier Ian, Kuhn Jens H, Dabisch Paul A, Mani Venkatesh
Аннотация
OBJECTIVES: The size of airborne particles may influence where they deposit in the respiratory tract, the infectious dose, and disease progression. Intranasal bolus administration is often used as a substitute for aerosol exposure. Inhalational and intranasal exposure routes are commonly used in hamster and guinea pig models of infectious diseases. The aim of the present study was to compare regional respiratory tract deposition of small- and large-particle aerosols to intranasal bolus administration.
METHODS: Golden hamsters were exposed to 2-deoxy-2-[fluorine-18]fluoro-D-glucose (18F-FDG)-radiolabeled aerosols with mass median aerodynamic diameters (MMADs) of 1.3 and 6.1 µm, and Hartley guinea pigs were exposed to 1.4 and 10.4 µm aerosols. Separately, hamsters and guinea pigs received bolus solutions of 18F-FDG (50 µL per naris) intranasally. Positron emission tomography-computed tomography (PET/CT) imaging was used to quantify the deposition of 18F-FDG in the respiratory tracts, including the oronasal cavities, tracheas/esophagi, and lungs.
RESULTS: Smaller particle aerosols deposited in the lungs more efficiently than larger particle aerosols or intranasal bolus administration. Large-particle aerosol and intranasal bolus administration resulted in greater oronasal deposition than small-particle aerosol.
CONCLUSIONS: PET/CT imaging-based quantification enabled novel characterization of particle deposition in rodents and supports an inverse relationship between aerodynamic particle size and pulmonary deposition. The regional distribution of intranasal bolus administered FDG more closely resembled large-particle aerosols than small-particle aerosols. These findings may aid in understanding the infectivity and pathogenicity of bioaerosols based on particle size and raise concern about substituting intranasal administration for aerosol exposure.
METHODS: Golden hamsters were exposed to 2-deoxy-2-[fluorine-18]fluoro-D-glucose (18F-FDG)-radiolabeled aerosols with mass median aerodynamic diameters (MMADs) of 1.3 and 6.1 µm, and Hartley guinea pigs were exposed to 1.4 and 10.4 µm aerosols. Separately, hamsters and guinea pigs received bolus solutions of 18F-FDG (50 µL per naris) intranasally. Positron emission tomography-computed tomography (PET/CT) imaging was used to quantify the deposition of 18F-FDG in the respiratory tracts, including the oronasal cavities, tracheas/esophagi, and lungs.
RESULTS: Smaller particle aerosols deposited in the lungs more efficiently than larger particle aerosols or intranasal bolus administration. Large-particle aerosol and intranasal bolus administration resulted in greater oronasal deposition than small-particle aerosol.
CONCLUSIONS: PET/CT imaging-based quantification enabled novel characterization of particle deposition in rodents and supports an inverse relationship between aerodynamic particle size and pulmonary deposition. The regional distribution of intranasal bolus administered FDG more closely resembled large-particle aerosols than small-particle aerosols. These findings may aid in understanding the infectivity and pathogenicity of bioaerosols based on particle size and raise concern about substituting intranasal administration for aerosol exposure.