Nature cell biology · 2022
Human induced pluripotent stem cell-derived salivary gland organoids model SARS-CoV-2 infection and replication.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
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Tanaka Junichi, Senpuku Hidenobu, Ogawa Miho, Yasuhara Rika, Ohnuma Shintaro, Takamatsu Koki, Watanabe Takashi, Mabuchi Yo, Nakamura Shiro, Ishida Shoko, Sadaoka Tomohiko, Takaki Takashi, Shirota Tatsuo, Shimane Toshikazu, Inoue Tomio, Sakai Takayoshi, Mori Munemasa, Tsuji Takashi, Saito Ichiro, Mishima Kenji
Аннотация
Salivary glands act as virus reservoirs in various infectious diseases and have been reported to be targeted by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, the mechanisms underlying infection and replication in salivary glands are still enigmatic due to the lack of proper in vitro models. Here, we show that human induced salivary glands (hiSGs) generated from human induced pluripotent stem cells can be infected with SARS-CoV-2. The hiSGs exhibit properties similar to those of embryonic salivary glands and are a valuable tool for the functional analysis of genes during development. Orthotopically transplanted hiSGs can be engrafted at a recipient site in mice and show a mature phenotype. In addition, we confirm SARS-CoV-2 infection and replication in hiSGs. SARS-CoV-2 derived from saliva in asymptomatic individuals may participate in the spread of the virus. hiSGs may be a promising model for investigating the role of salivary glands as a virus reservoir.