Effect of Matrix Metalloproteinase Inhibitors on the Bonding Durability of Nanocomposite Resin to Caries-affected Dentin: An In Vitro Study.

AIM: This in vitro study evaluated the microtensile bond strength (µTBS) of two nanohybrid resin composites to caries-affected dentin (CAD) after pretreatment with different hesperidin (HPN) concentrations, before and after thermocycling.

MATERIALS AND

METHODS

Eighty extracted human molars were assigned to two groups by composite type (Filtek Z250 XT, Grandio; n = 40 each) and subdivided into four pretreatment protocols (n = 10): control (no treatment), 0.5%, 1%, or 2% HPN. Half of the specimens in each subgroup were subjected to thermocycling. Microtensile bond strength was measured using a universal testing machine. Data were analyzed using two-way and three-way ANOVA followed by post hoc tests (α = 0.05).

RESULTS

Bond strength was significantly affected by HPN concentration and thermocycling. Non-aged Filtek and Grandio showed higher values (up to 39.90 ± 10.67 and 45.87 ± 13.37 MPa, respectively), while aging reduced strength in specific subgroups (Filtek no-treatment: p = 0.016; Grandio 0.5% HPN: p = 0.004; 2% HPN: p = 0.033). Overall, 0.5% HPN maintained better adhesive performance after aging, whereas higher concentrations showed diminished stability. Failure mode analysis supported these trends, with higher bond strength linked to cohesive/mixed failures.

CONCLUSION

Pretreatment with 0.5% HPN significantly improved bond strength and durability to CAD, while higher concentrations reduced performance, especially after thermocycling.

CLINICAL SIGNIFICANCE: Applying 0.5% HPN as a dentin pretreatment may enhance the longevity of composite restorations in cases involving CAD. How to cite this article: Abdel-Salam FD, Sheta N, Eid EG. Effect of Matrix Metalloproteinase Inhibitors on the Bonding Durability of Nanocomposite Resin to Caries-affected Dentin: An In Vitro Study. J Contemp Dent Pract 2025;26(11):1096-1102.