Journal of clinical laboratory analysis · 2026
Immune Imbalance in Sickle Cell Anemia: Flow Cytometric Insights Into Regulatory T Cells and Neutrophil Dynamics.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Ölçüoğlu Rukiye, Tanrıkulu Funda, Kılınç Sevtap, Çakmak Eda, Asma Süheyl, Boğa Can, Özdoğu Hakan, Pamuk İlknur
Аннотация
OBJECTIVE: To investigate immunophenotypic alterations in regulatory T cells (Tregs) and neutrophil dynamics in adult sickle cell anemia (SCA) patients during painful crises and steady state.
METHODS: Ninety-three participants were included: 17 SCA patients in painful crisis, 27 in steady state, and 49 healthy controls. Flow cytometry was used to assess CD3+CD4+CD25+FoxP3+ Tregs and related subsets. Hematological parameters were evaluated by complete blood counts. Statistical analyses included group comparisons, multiple regression, and ROC curve analysis.
RESULTS: SCA patients exhibited significant reductions in CD25+ and CD4+ T cell subsets, despite preserved FoxP3+ Treg frequencies. White blood cell and neutrophil counts were elevated, especially during crisis. Neutrophil and lymphocyte percentages significantly predicted T cell subset levels. ROC analysis identified CD3+ and CD4+ percentages as strong classifiers of disease state.
CONCLUSION: Despite numerical preservation of FoxP3+ Tregs, SCA is marked by impaired T cell activation and sustained innate immune activation. These immune shifts may relate to but do not directly determine end-organ complications. Functional assays and longitudinal studies are needed to elucidate Treg competence, hydroxyurea effects, and age-related immune changes in SCA.
METHODS: Ninety-three participants were included: 17 SCA patients in painful crisis, 27 in steady state, and 49 healthy controls. Flow cytometry was used to assess CD3+CD4+CD25+FoxP3+ Tregs and related subsets. Hematological parameters were evaluated by complete blood counts. Statistical analyses included group comparisons, multiple regression, and ROC curve analysis.
RESULTS: SCA patients exhibited significant reductions in CD25+ and CD4+ T cell subsets, despite preserved FoxP3+ Treg frequencies. White blood cell and neutrophil counts were elevated, especially during crisis. Neutrophil and lymphocyte percentages significantly predicted T cell subset levels. ROC analysis identified CD3+ and CD4+ percentages as strong classifiers of disease state.
CONCLUSION: Despite numerical preservation of FoxP3+ Tregs, SCA is marked by impaired T cell activation and sustained innate immune activation. These immune shifts may relate to but do not directly determine end-organ complications. Functional assays and longitudinal studies are needed to elucidate Treg competence, hydroxyurea effects, and age-related immune changes in SCA.