[The detection of cytomegalovirus DNA by the polymerase chain reaction in different biological materials in patients with organ allografts].

Ninety-eight patients with organ allografts were examined using the polymerase chain reaction (PCR). Cytomegalovirus (CMV) DNA was not found in the blood of any of 67 blood donors. At least 85% patients with CMV DNA in the plasma developed CMV disease. CMV DNA was detected in leukocytes of 22 (40.7%) out of 54 patients with negative results of analysis for CMV DNA in the plasma; 16 (29.6%) of these 22 presented with clinical symptoms of CMV infection by the moment of investigation. Results of PCR analysis of blood, urine, and saliva specimens of 30 patients examined did not always coincide. DNA isolated from the urine can contain components inhibiting DNA polymerase. Blood leukocytes or whole blood are the most suitable for the diagnosis of active and symptomatic CMV-infection.