styMdtM gene deletion with modified λ Red recombineering approach: Implications for multidrug resistance in Salmonella Typhi.

styMdtM is a multidrug efflux transporter of Salmonella Typhi. In the present study, a modified λ Red recombineering approach using shorter flanking arms (ca. 50 bp) was used to generate an styMdtM gene deletion in the S. Typhi genome. This approach worked efficiently to knockout the gene and the ensuing S. Typhi strain was considerably more sensitive to antibiotics.