A successful case of assisted reproduction after contamination of the culture medium with Escherichia coli.

INTRODUCTION

We report a case of successful pregnancy after in vitro fertilization culture (IVF) medium contaminated with Escherichia coli (E. coli).

CASE REPORT: To remove E. coli, oocytes were isolated in a PVP-ICSI medium and then in a culture medium supplemented with gentamicin. We selected two embryos that were vitrified and frozen. In the next natural cycle, we performed the embryo transfer of 2 embryos.

RESULTS

Only one pregnancy was uncomplicated until term and was completed by the live birth of a healthy baby. The child's subsequent development until the date of publication of this article is normal.

CONCLUSIONS

In cases of E. coli contamination of the IVF culture medium, specific laboratory methods allow the procedure to be continued, and a healthy baby can be obtained.