Future microbiology · 2016
Evaluation of antibacterial and antibiofilm mechanisms by usnic acid against methicillin-resistant Staphylococcus aureus.
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Pompilio Arianna, Riviello Antonella, Crocetta Valentina, Di Giuseppe Fabrizio, Pomponio Stefano, Sulpizio Marilisa, Di Ilio Carmine, Angelucci Stefania, Barone Luana, Di Giulio Andrea, Di Bonaventura Giovanni
Аннотация
AIM: To evaluate the antibacterial and antibiofilm mechanisms of usnic acid (USN) against methicillin-resistant Staphylococcus aureus from cystic fibrosis patients.
MATERIALS & METHODS: The effects exerted by USN at subinhibitory concentrations on S. aureus Sa3 strain was evaluated by proteomic, real-time PCR and electron microscopy analyses.
RESULTS & CONCLUSION: Proteomic analysis showed that USN caused damage in peptidoglycan synthesis, as confirmed by microscopy. Real-time PCR analysis showed that antibiofilm activity of USN is mainly due to impaired adhesion to the host matrix binding proteins, and decreasing lipase and thermonuclease expression. Our data show that USN exerts anti-staphylococcal effects through multitarget inhibitory effects, thus confirming the rationale for considering it 'lead compound' for the treatment of cystic fibrosis infections.
MATERIALS & METHODS: The effects exerted by USN at subinhibitory concentrations on S. aureus Sa3 strain was evaluated by proteomic, real-time PCR and electron microscopy analyses.
RESULTS & CONCLUSION: Proteomic analysis showed that USN caused damage in peptidoglycan synthesis, as confirmed by microscopy. Real-time PCR analysis showed that antibiofilm activity of USN is mainly due to impaired adhesion to the host matrix binding proteins, and decreasing lipase and thermonuclease expression. Our data show that USN exerts anti-staphylococcal effects through multitarget inhibitory effects, thus confirming the rationale for considering it 'lead compound' for the treatment of cystic fibrosis infections.
Предметные рубрики MeSH
Adhesins, BacterialAnti-Bacterial AgentsBacterial ProteinsBenzofuransBiofilmsCarrier ProteinsCell MembraneCell SurvivalColony Count, MicrobialCystic FibrosisDNA, BacterialDown-RegulationLipaseMethicillin-Resistant Staphylococcus aureusMicrobial Sensitivity TestsMicrobial ViabilityMicroscopy, Electron, ScanningMicroscopy, Electron, TransmissionPeptidoglycanPropidiumProtein Interaction MapsProteomicsReal-Time Polymerase Chain ReactionSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationStaphylococcal InfectionsTime FactorsVirulence