Expression of mesenger RNA for transforming growth factor-beta 1 in bovine trabecular meshwork.

PURPOSE: To investigate the relationship between transforming growth factor-beta 1 (TGF-beta 1) and primary open-angle glaucoma, we have determined whether trabecular tissues have the expression of messenger RNA for TGF-beta 1.

METHODS

Total RNA of 24 newborn bovine trabecular tissue were extracted by Guanidine isothiocyanate method. The TGF-beta 33 plasmid was brought into E. colibacillius HB101 and amplificated. After Bam HI endolase degradation and label with alpha-32p-dATP the RNA was hybridized with the cDNA (complementary DNA) probe and examined by autoradiography.

RESULTS

The presence of mRNA for TGF-beta 1 in bovine trabecular meshwork was confirmed.

CONCLUSIONS

The TGF-beta 1 present in normal aqueous humor must be at least partly derived from the trabecular meshwork. It offered a basis for understanding the relationship between abnormal synthesis, activation and clearance of TGF-beta 1 and the pathogenesis of primary open-angle glaucoma (POAG) in molecular biology.