International journal of biological macromolecules · 2026
Edible packaging fucoidan/ovalbumin encapsulation co-delivery of indigo and Indirubin nanoparticles: Preparation, characterization, and preliminary mechanism of action against Helicobacter pylori in vitro.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Lin Fengli, Chen Meiyun, Li Yanqi, Li Haonan, Cui Jie, Shi Xiaoyan, Xiao He, Jia Junwei, Wei Ruixia, Zhang Guimin, Yao Meicun, Feng Zhong
Аннотация
BACKGROUND AND OBJECTIVE: Indigo and indirubin are the main active components of the traditional Chinese medicine Qingdai, known for their heat-clearing, detoxifying, antibacterial, and anti-inflammatory effects. Indirubin has already been applied clinically with proven safety. Both compounds show potential against drug-resistant Helicobacter pylori infection; however, their strong hydrophobicity limits further application. This study aimed to construct food-grade self-assembled nanoparticles using electrostatic and hydrophobic interactions between ovalbumin and fucoidan to efficiently encapsulate indigo and indirubin, thereby improving their solubility and evaluating the in vitro anti- Helicobacter pylori activity and underlying mechanisms of the three formulations.
RESULTS: At a carrier concentration of 0.75 mg/mL and a drug loading concentration of 40 μg/mL, the nanoparticles exhibited optimal stability, with an encapsulation efficiency of 68.64% and a loading capacity of 3.66%. Indigo, indirubin, and their nanoparticles showed significant inhibitory and bactericidal effects against both sensitive and drug-resistant Helicobacter pylori strains, with minimum inhibitory concentration ranges of 2.5-5, 5-32, and 2.5-5 μg/mL, respectively. The nanoparticles demonstrated superior efficacy and exhibited synergistic or additive effects when combined with antibiotics. The underlying mechanisms involved disruption of bacterial structures; downregulation of virulence genes related to flagella, adhesion, urease, and VacA; inhibition of urease activity and CagA expression; and interference with key metabolic pathways.
CONCLUSION: Indigo and indirubin exhibit significant anti-Helicobacter pylori activity, and their encapsulation within ovalbumin/fucoidan self-assembled nanoparticles markedly enhances both solubility and anti- Helicobacter pylori efficacy. This approach provides an experimental basis for the development of novel natural therapies targeting Helicobacter pylori infection.
RESULTS: At a carrier concentration of 0.75 mg/mL and a drug loading concentration of 40 μg/mL, the nanoparticles exhibited optimal stability, with an encapsulation efficiency of 68.64% and a loading capacity of 3.66%. Indigo, indirubin, and their nanoparticles showed significant inhibitory and bactericidal effects against both sensitive and drug-resistant Helicobacter pylori strains, with minimum inhibitory concentration ranges of 2.5-5, 5-32, and 2.5-5 μg/mL, respectively. The nanoparticles demonstrated superior efficacy and exhibited synergistic or additive effects when combined with antibiotics. The underlying mechanisms involved disruption of bacterial structures; downregulation of virulence genes related to flagella, adhesion, urease, and VacA; inhibition of urease activity and CagA expression; and interference with key metabolic pathways.
CONCLUSION: Indigo and indirubin exhibit significant anti-Helicobacter pylori activity, and their encapsulation within ovalbumin/fucoidan self-assembled nanoparticles markedly enhances both solubility and anti- Helicobacter pylori efficacy. This approach provides an experimental basis for the development of novel natural therapies targeting Helicobacter pylori infection.